https://scholarlywiki.org/index.php?action=history&feed=atom&title=Physics%3A4Pi_STED_microscopy 2026-05-23T02:35:44Z Revision history for this page on the wiki MediaWiki 1.44.5 https://scholarlywiki.org/index.php?title=Physics:4Pi_STED_microscopy&diff=2443&oldid=prev 2022-04-18T21:20:10Z

<p>update</p> <p><b>New page</b></p><div>{{Context|date=March 2013}}<br /> <br /> The &#039;&#039;&#039;4Pi-STED-microscope&#039;&#039;&#039; is the result of combining the two unrelated concepts of [[Biology:STED microscopy|Stimulated emission depletion (STED) microscopy]] and 4Pi-microscopy. Here, a fluorescent sample is placed in the common [[Physics:Focus (optics)|focus]] of two opposing [[Physics:Lens (optics)|lenses]], but [[Physics:Excited state|excitation]] and [[Social:Detection|detection]] are performed through a single lens (4Pi mode A). The green excitation [[Physics:Pulse|pulse]] is immediately followed by a red STED-pulse, which enters the focal region through both lenses inducing stimulated [[Physics:Emission spectrum|emission]] of the excited fluorescent [[Physics:Molecule|molecule]]s to the [[Physics:Ground state|ground state]]. To permit fluorescence emission from the center but suppress it from neighbouring regions it is useful to phase shift the STED beam to have a minimum at the center.<br /> <br /> ==External links==<br /> * [http://www.mpibpc.gwdg.de/abteilungen/200/4Pi-STED.htm Technical details]<br /> <br /> [[Category:Microscopy]]<br /> <br /> <br /> <br /> {{Sourceattribution|4Pi STED microscopy}}</div>

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